Abstract for Flexi^® Vector Systems Technical Manual

Part# TM254
Printed in USA. Revised 8/09.
Instructions for Use of Products C8640 C8820 & C9320.

The Flexi^® Vector Systems use two rare-cutting restriction enzymes, Sgf I and Pme I, in a simple, yet powerful, directional cloning method for protein-coding sequences. These systems provide a rapid, efficient and high-fidelity way to transfer these sequences between a variety of Flexi^® Vectors. The Flexi^® Vectors contain various expression or peptide tag options to enable expression of native or fusion proteins to study protein structure and function, as well as protein-protein interactions. All Flexi^® Vectors carry the lethal barnase gene, which is replaced by the DNA fragment of interest and acts as a positive selection for successful ligation of the insert.