Abstract for Flexi^® Rabbit Reticulocyte Lysate System

Part# TB127
Printed in USA. Revised 3/09.
Instructions for Use of Product L4540.

The rabbit reticulocyte in vitro translation system is widely used to identify mRNA species and characterize their products. This eukaryotic system has been reported to post-translationally modify proteins via phosphorylation, acetylation and isoprenylation. Signal peptide cleavage and core glycosylation also can be achieved by the rabbit reticulocyte lysate system with the addition of Canine Pancreatic Microsomal Membranes. The Flexi^® Rabbit Reticulocyte Lysate System provides greater flexibility of reaction conditions than standard rabbit reticulocyte lysate systems. As with the standard Rabbit Reticulocyte Lysate, the Flexi^® Rabbit Reticulocyte Lysate is optimized for translation by addition of:
• Hemin, to prevent inhibition of initiation factor eIF-2α
• An energy-generating system consisting of pretested phosphocreatine kinase and phosphocreatine
• Calf liver tRNAs, to balance the accepting tRNA populations thus optimizing codon usage and expanding
   range of mRNAs that can be translated efficiently
• Micrococcal nuclease, to eliminate endogenous mRNA, thus reducing background translation.