|
Download
article
191kb
pdf? |
|
Technically Speaking: TNT® Rabbit Reticulocyte Lysate Systems–Easy Protein Expression
Alyssa TenHarmsel, Ph.D.
Promega Corporation
The TNT® Coupled Reticulocyte Lysate Systems offer a one-tube, coupled transcription/translation approach
to protein expression. These systems reduce the time required to obtain
in vitro translation results by incorporating transcription directly in
the translation mix. There are several factors to be considered when
choosing a TNT® System. These include the starting template, the type of
protein to be translated, the preferred detection method, the desired
reaction format and the intended downstream applications. Here we
discuss how to decide which TNT® System is best for you.
|
