In vitro Expression Cloning Using the TNT®
Coupled Reticulocyte Lysate System
By Greg Beckler
Promega Corporation
Author's Note: This article
summarizes information previously published in peer-reviewed journal articles that
featured the use of Promega's reagents and systems in novel experiments in the life
sciences. The publisher of the original journal article has granted Promega permission to
reproduce the figure shown here.
The in vitro expression cloning or IVEC technique is a systematic and broadly applicable
method for the genetic characterization and biochemical screening of genes and cognate
proteins. IVEC uses the TNT®
Coupled Reticulocyte Lysate System to express
proteins from small pools of cDNA libraries, greatly simplifying the process of isolating
genes that encode proteins identified by a particular biochemical function or
characteristic. Here, we summarize the experimental procedure and suggest possible uses
for the technology.
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